Protein rich agro industrial wastes constitute valuable source for microbial cultivation and large scale extracellular protease production. An attempt was made to investigate protease production by Gymnascella dankaliensis (Castellani) Currah FGCC P 134 on various agro based waste under solid-state fermentation. Interestingly, among different substrate tried, wheat bran proved superior supported 299±6.11 U/g DMS protease activity after 96 h of incubation, which may result in a significant reduction in the cost of medium. To study the maximum enzyme production the process parameters of fermentation where optimized. Maximum protease production was obtained with an initial moisture content of 40%, on incubation temperature (32±2°C), pH (6.5), spore concentration ~ 4.16 x 106 of 16 old inoculum.
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